The following anonymous DOD document omits some relevant facts and misrepresents several studies regarding anthrax vaccine safety.  I have provided additional information, comments and references.  My remarks are in bold.

 

Meryl Nass, MD

March 20, 2007

 

Surveillance Program for Short-term Health Effects of AVA.

 

To monitor rare or unexpected adverse events associated in time to any vaccine, DoD health-care providers have participated in the Vaccine Adverse Event Reporting System (VAERS), since its inception in 1990.

 

Military medical providers have been loathe to file anthrax vaccine adverse event reports due to perceived adverse effects on their careers if they do so.  This probably stems from the instruction to medical providers NOT to report adverse events unless the patient missed more than 24 hours of work, was hospitalized, or contamination of an entire lot of vaccine was suspected.[1]

 

Therefore, after changing this guidance in response to Congressional testimony about the failures to file,[2] repeated instructions to file these reports by top military leaders have been issued to providers in 1999,[3] 2000[4] and 2004.[5]  This would suggest that military medical providers continued to fail to file the reports.

 

For anthrax vaccine, each VAERS report involving anthrax vaccine between 1998 and 2001 was reviewed by an independent panel of civilian physicians. This panel detected no patterns of unexpected adverse events related to anthrax vaccination. Despite the extensive body of knowledge regarding the safety of anthrax vaccine, safety monitoring continues, as is prudent for all vaccines and medications.

 

The independent panel wrote two published papers on their analysis.  Contrary to the claim that no patterns of unexpected adverse events were found, the second paper,[6] which reviewed more cases than the first, found several patterns of Gulf War Illness-like reports to VAERS at 2 to 3 times the expected rate in anthrax vaccine recipients.

 

As directed by the 2001 National Defense Authorization Act, the DoD and the Centers for Disease Control and Prevention have collaborated to establish a Vaccine Healthcare Center Network, a system for enhancing the monitoring of vaccine associated adverse events experienced by uniformed services members. This monitoring assists in determining the need for immunization safety assessments and possible alternative management strategies to preserve deployability of those who experience adverse reactions.

 

The network functions as an allergy-immunology clinical evaluation unit specializing in vaccine-associated adverse events and is accessible to DoD beneficiaries either directly or on a referral basis. The VHC maintains a capability to support a comprehensive vaccine safety assessment program. The scope of services is broad and ranges from surveillance through enhanced vaccine adverse events reporting and case management of complex adverse events. The Network assists in data collection and standardization in support of improvement of the vaccine adverse events reporting system (VAERS) for all required vaccines including the anthrax vaccine. Emphasis is placed on standardization of clinical and educational programs that focus on healthcare provider and beneficiary understanding of immunizations and vaccine safety.  Clinical research partnerships have been developed to validate clinical guidelines and support improvements in vaccine healthcare delivery.

 

The discussion of the VHC network above is correct.  However, several points should be added.  First, many more clinics were initially envisioned, but the number of clinics created stopped at four.  Second, funding for this network has been threatened during the past two funding cycles and remains in question.  Staff have resigned, and there have been absent medical directors at some clinics, which are primarily staffed by nurse practitioners.  Third, although the mission of the VHCs was, in part, to perform research on vaccine reactions, there are no published papers or research reports available from the VHC network.  No ongoing research projects have been announced to the public.  Fourth, although at least 1,700 complete reviews of patients reporting illnesses related to anthrax vaccine have been performed,[7] the VHCs have failed to provide information on the types of reactions they have evaluated, other contributing factors, their thoughts on causality and treatment, which would greatly assist other medical providers. 

 

Surveillance Program for Long-term Health Effects of AVA.

 

There are no known long-term patterns of side effects from the anthrax vaccine, based on an ongoing series of studies at Fort Detrick, Maryland, and elsewhere. Reports in this series were published in 1958, 1965, 1974, 2001, and 2004. 

 

The reports from 1958, 1965 and 1974 were of workers who received many vaccines at Fort Detrick, and were confounded by the Òhealthy workerÓ effect and the inability to separate the effects of anthrax vaccine from other vaccines.  These studies did show several abnormalities in blood tests of the multiply-vaccinated cohort, but their clinical effect was uncertain.

 

Regarding the 2001 report by Pittman et al at Fort Detrick:

 

Passive surveillance data on 1,583 Fort Detrick employees who had received a total of 10,722 doses of anthrax vaccine between 1973 and 1999 were reviewed.[8]  Two hundred seventy-three persons received 10 or more doses.  Other vaccines were also given to this population.  Employees visited the clinic after 3.6% of anthrax inoculations for local reactions, and after 1% of inoculations for systemic reactions.  One female developed an acute demyelinating disorder 8 days after the second anthrax vaccination.  Reaction rates were significantly affected by lot and gender, with women having more reported reactions than men.  A first reaction increased the odds of presenting with later reactions.

 

The authors concluded, ÒA prospective, randomized, placebo-controlled study to actively examine reactogenicity is needed to more completely define the extent and nature of reactions associated with receipt of AVA in humans, as well as to confirm the gender and lot differences in local reaction rates.Ó

 

Despite the extensive body of knowledge regarding the safety of anthrax vaccine, safety monitoring continues, as is prudent for all vaccines and medications.

 

Details of each study appear below. The studies include:

 

1. Disability Study. The Department of Defense (DoD) studied whether anthrax immunized Service members have higher rates of evaluation for disability discharge from March 1998 to February 2002. The study results were published in the Journal of Occupational and Environmental Medicine in 2004.1 This study determined the rates of disability evaluation among 716,833 Service members followed for 4.25 years 154,456 of whom received at least one dose of anthrax vaccine. The study compared the risk of disability evaluation in Service members (immunized vs. unimmunized) accounting for occupation and sociodemographics (age, gender, race/ethnicity, marital status and education). The results showed that anthrax immunization did not increase the risk of either temporary or permanent disability among the Service members studied.

 

This study was performed by a contractor for the Anthrax Vaccine Agency, which provided all the raw data.  There was no independent oversight or review to confirm validity of the data provided to the contractor or the contractorÕs analysis.  The second author was the Director of the Anthrax Vaccine Agency.

 

2. Optic Neuritis Study2 The optic neuritis study was a matched case-control study among military personnel conducted from January 1998 through December 2003.  The study determined whether a statistical association exists between vaccine exposures and optic neuritis within 6-, 12-, and 18-week study intervals. A total of 1131 cases of optic neuritis and 3393 controls were matched by sex, military component, and deployment status. No statistically significant associations between optic neuritis and anthrax vaccine were observed for any of the three study intervals. The same was true for the other vaccines evaluated (smallpox, hepatitis B and influenza). The authors concluded that there is no association between optic neuritis and receipt of anthrax, smallpox, hepatitis B, or influenza vaccinations in the US military, whether administered alone or in combination.

 

This study[9] employed a low power, case-control method to explore the role of vaccinations in optic neuritis.  Soldiers had to be diagnosed with optic neuritis within 18 weeks post-vaccination to be included as cases. Since 2003, soldiers have started the anthrax series just before 6-12 month deployments to Afghanistan and Iraq.  Thus CDCÕs inclusion criteria required most cases to be diagnosed while on deployment.  Since diagnosis requires an ophthalmologist evaluation, and soldiers have limited access to these specialists in a war zone,[10] it is likely that only the most severe cases met the inclusion criteria.  Controls were not properly matched to cases.  The authors concluded that optic neuritis is unrelated to anthrax vaccine.  Although the DMSS provided the data, the studyÕs design undercut this conclusion, as pointed out in a published Letter I submitted to the editor.[11]

 

3. Military Hospitalizations Study.3 This study included 170,723 active duty anthrax-vaccinated service members deployed during the time period January 1, 1998 to December 21, 2001. This study evaluated hospitalizations for any-cause, 14 broad International Classification of Disease (ICD-9) diagnostic categories, autoimmune organ specific and organ non-specific hospitalizations, and asthma. The results of the study showed that anthrax vaccination was associated with significantly fewer hospitalizations for any cause, diseases of the blood and blood forming organs, and diseases of the respiratory system.

 

The fact that anthrax vaccination was associated with significantly fewer hospitalizations for any cause is a reflection of the well-known fact that deploying servicemembers (who are vaccinated in preparation for deployment) are a healthier group than nondeploying members.  If they werenÕt, these results would imply that anthrax vaccine prevents all sorts of diseases—and no one is claiming that.  The better analysis is to compare the same group pre and post vaccination, to avoid confounding by the Òhealthy warriorÓ effect.  When this was done, hospitalizations for a number of illnesses (thyroid cancer, carcinoma in situ of the breast and genitourinary system, diabetes, psychoses, depression, other forms of ischemic heart disease, thrombophlebitis, asthma, other disorders of intestine, noninflammatory disorders of the cervix, etc.)[12] were greater in the vaccinated group.  Two Institute of Medicine committees have recommended since 2002 that this be explored further[13] [14]—but it has not happened.

 

4. Female Reproductive Health Study4 This cohort study was designed to determine whether receipt of anthrax vaccine by reproductive-aged women has an effect on pregnancy rates. The study evaluated a total of 4092 women (ages 17-44) stationed at Fort Stewart, GA from January 1999 to March 2000. Results showed there were 513 pregnancies in the women studied with 385 following at least one dose of anthrax vaccine. After comparing pregnancy and birth rates between vaccinated and unvaccinated women the authors concluded that anthrax vaccination had no effect on pregnancy and birth rates.

 

The Naval Environmental Health Research Center performed a study comparing birth defect rates in offspring of women who inadvertently received anthrax vaccine during the first trimester, to birth defect rates in women who got the vaccine at any other time.[15] The results showed a "slight but significant increase" in birth defects in the first trimester-vaccinated women. Ryan presented these results to several groups in late 2001 and early 2002, including the FDA and Institute of Medicine committee on the safety and efficacy of anthrax vaccine.

 

The research results, though unpublished, led to several actions, according to the IOM report:[16]

 

1.  December, 2001:  The informed consent document for CDCÕs IND trial of anthrax vaccine in those exposed to the anthrax letters added a pregnancy warning.

 

2.  Jan 16, 2002:  The Assistant Secretary of Defense for Health Affairs issued a memorandum[17] and press release[18] demanding the services check whether women were pregnant before vaccinating, coordinate with the ArmyÕs Anthrax Program, and provide him Òwithin 14 days of this memo, your ServiceÕs plan to strengthen the screening of females of childbearing age with careful questioning or other positive efforts, potentially including pregnancy testing when appropriate, to prevent the administration of vaccine to pregnant women.Ó

 

3.  January 31, 2002:  FDA approved both the new anthrax manufacturing facility and a new product label, and released stockpiled anthrax vaccine.  The vaccineÕs pregnancy risk category was changed from category C (ÒRisk cannot be ruled outÓ) to D (ÒPositive evidence of riskÓ).

 

However, on February 15, 2002 the Morbidity and Mortality Weekly Report wrote that there were concerns regarding the accuracy of computerized medical records used in the Navy study, and a paper review, taking several months, would be performed for clarification.[19]  The March 2002 IOM report stated, ÒBecause of the importance of this issue, the study investigators are working rapidly to validate both exposures and outcomes using primary data sources.Ó  However, the Navy study of birth defects remains unpublished, five years later.

 

An Army study of pregnancy rates following anthrax vaccine was published in March 2002. [20] This study used computerized records, but performed no verification with paper records.  This study excluded high-risk pregnancies that were referred outside the military hospital. Twenty-five pregnancies were lost to follow-up.  In 385 pregnancies following any anthrax inoculations, of which some were lost to follow-up, there were 15 known birth defects (a 3.9% rate or greater) compared to a birth defect rate in the US overall of approximately 3% and one military rate of 3.2%.[21]  The pregnancy rate was the same in immunized and unimmunized women. Although the authors said, ÒThis study did not have sufficient power to detect adverse birth outcomes,Ó and ignored the relatively high birth defect rate, the study is cited as evidence that anthrax vaccine has no effect on reproductive health by the US military and by a surrogate of the manufacturer.[22] [23]

 

 

5. Male Fertility Study5 This study evaluated the impact of anthrax vaccination on sperm and clinical pregnancy rates among couples in which the man had or had not been exposed to anthrax vaccination. From October 1999 to December 2003 254 males who had received anthrax vaccination and 791 unvaccinated males enrolled in the Walter Reed Army Medical Center Assisted Reproductive Technologies program were evaluated. To evaluate the impact of anthrax vaccination on male fertility, the study compared characteristics of the men, the couples, the fertilization, and the outcome of fertilization. The investigators conclusion was that exposure to the anthrax vaccine by males undergoing assisted reproduction did not negatively impact semen parameters, fertilization rate, embryo quality, or clinical pregnancy rates.

 

They compared anthrax-vaccinated males from infertile couples to unvaccinated males from infertile couples.  This fails to answer the question of whether anthrax vaccine causes male fertility problems.

 

6. Tripler Army Medical Center Healthcare Worker Study6 This study evaluated a broad range of health effects in a cohort of 601 healthcare workers immunized with anthrax vaccine. The healthcare workers surveyed were a highly educated, medically experienced population, familiar with adverse events, with easy access to health care. The findings of this study showed that regardless of gender, most adverse events after vaccination were mild and self-limited. There was no evidence that anthrax vaccinated healthcare workers had higher rates of either outpatient health visits, hospitalizations, or a change in self-reported health and wellness when compared to unimmunized individuals.

 

According to the principal investigator, "The objectives were to provide active surveillance of self-reported side effects and the duration of symptoms." [24]  Soldiers were asked to complete a questionnaire about symptoms that developed after their last anthrax vaccination when they presented for a subsequent inoculation or within two weeks of an inoculation.

 

Three significant neurological events were described.  One case of multiple sclerosis developed early in the study.  A neonatologist developed upper extremity weakness and tremor associated with a CPK level over 1000.  A pediatric cardiologist developed numbness and fasciculations, suggesting brachial plexopathy, which was said to later resolve.

 

Despite the studyÕs original intention to actively follow adverse events longitudinally, the exit questionnaire chosen was not designed to capture specific information about vaccine-related adverse events and their duration, and made no reference to anthrax vaccine.  The published paper fails to provide the duration of systemic reactions. It states that only local reactions could be linked to vaccination, and dismisses vaccine causality for other reactions, without providing a rationale.  It fails to explore why 0.5% of participants developed a serious neurologic condition in close temporal relationship to vaccination, or why immunized women who completed the trial were 4.4 times as likely to report poor or fair health as an unvaccinated observational group of women at the same base.

 

Other problems with this study include the fact that although 603 persons were enrolled and originally reported on,[25] the published paper mentions only 601 subjects.  By the time the trial was completed, at eighteen months, over half the participants had been lost to follow-up.

 

The abstract states that local reactions occurred more often in women, but neglects to mention that systemic reactions did also, at 1.5 – 2 times the rate of men.   Although the published paper notes that one reason for enrollees to drop out was pregnancy, it omits mention of eleven women who became pregnant after starting the vaccine series, and does not report pregnancy outcomes.

 

 

7. Flight Physicals Study (Forthcoming). This study retrospectively compared data from periodic examinations of US Army aircrew members between 1998 and 2005. The study included 6,820 immunized and 4,145 unimmunized personnel. For each variable evaluated there was no association between anthrax immunization and any clinically relevant change in a physiologic parameter.

 

Not available for review.

 

8. The Millennium Cohort Study (Ongoing).  The Millennium Cohort Study was designed to evaluate the long-term health effects of military service, specifically deployments. The Department of Defense realized after the 1991 Gulf War that there was a need to collect more information about the long-term health of service members. The Millennium Cohort Study was designed to address that critical need, and the study was underway by 2001.  Funded by the Department of Defense, and supported by military, Department of Veterans Affairs, and civilian researchers, almost 110,000 people have already participated in this groundbreaking study. As force health protection continues to be a priority for the future of the United States military, the Millennium Cohort Study will be providing a crucial step towards enhancing the long-term health of military service members. Additional information available from: www.millenniumcohort.org

Not available for review.

 

References.

 

1. Sulsky SI, Grabenstein JD, Deldos RG. Disability among U.S. Army personnel vaccinated against anthrax. J Occup Environ Med 2004;46:1065-1075.

 

2. Payne DC, Rose CE, Kerrison J, Aranas A, Duderstadt S, McNeil MM. Anthrax vaccination and risk of optic neuritis in the United States military, 1998-2003. Arch Neurol 2006;63:871-5.

 

3. Wells TA, Sato PA, Smith TC, Wang LZ, Reed RJ, Ryan MAK. Military hospitalizations among deployed US service members following anthrax vaccination, 1998-2001. Huamn Vaccine 2006;2(2):54-59.

 

4. Weisen AR, Littell CT. Relationship between prepregnancy anthrax vaccination and pregnancy and birth outcomes among US Army women. JAMA 2002;287(12):1556-60.

 

5. Catherino WH, Levi A, Kao TC, Leondires MP, McKeeby J, Segars JH. Anthrax vaccine does not affect semen parameters, embryo quality, or pregnancy outcomes in couples with a vaccinated male military service member. Fertil Steril 2005;83:480-3.

 

6. Wasserman GM, Grabenstein JD, Pittman PR, Rubertone MV, Gibbs PP, Wang LZ, Golder LG. Analysis of adverse events after anthrax vaccination in US Army medical personnel. J of Occup Environ Med 2003;45:222-33.

 

 

 



[1] Instructions from Secretary of the Navy:  SECNAVINST 6230.4. April 29, 1998.  ANNEX C TO ENCLOSURE (1). PAGE C-5.  It stated, ÒReport all adverse vaccine reactions resulting in hospitalizations or time lost from duty (more than 24 hours), using the Health and Human Services Vaccine Adverse Events form.  Other reactions will not be reported unless contamination of lots is suspected.Ó

[2] Hearing, Committee on Government Reform. Subcommittee on National Security, Veterans Affairs and International Relations. Anthrax Vaccine Adverse Reactions. July 21, 1999.

[3] Bailey S. (Assistant secretary of defense for Health Affairs). Memorandum for Service Surgeons General. Subject: Policy for reporting adverse events associated with anthrax vaccine. October 15, 1999.

[4] Clinton JJ. (Acting Assistant Secretary of Defense). Memorandum for Service Surgeons General. Subject: Reactions to the anthrax vaccine. October 6, 2000.

[5] Peake JB. (Lieutenant General Commanding). Memorandum for Commanders, regional Medical Commands. Subject: Learning from adverse events after vaccination-ACTION MEMORANDUM. FEBRUARY 10, 2004.

 

[6] Sever JL, Brenner AI, Gale AD, Lyle JM, Moulton LH, Ward BJ, West DJ. Safety of anthrax vaccine: an expanded review and evaluation of adverse events reported to the Vaccine Adverse Event Reporting System (VAERS).Pharmacoepidemiol Drug Safety. 2004 Dec;13(12):825-40.

 

 

 

[7] Testimony of David Hrncir, MD, Director, Wilford Hall Vaccine Healthcare Center. Court martial trial transcript of Snr Airman Nathaniel Torquato, Travis Air Force Base. January 26, 2006.  LTC Nancy J Paul, presiding.

[8] Pittman PR, Gibbs PH, Cannon TL et al. Anthrax vaccine: Short-term safety experience in humans. Vaccine 2001; 20: 972-8.

[9] Payne DC, Rose CE Jr, Kerrison J, Aranas A, Duderstadt S, McNeil MM. Anthrax vaccination and risk of optic neuritis in the United States military, 1998-2003. Arch Neurol. 2006 Jun;63(6):871-5.

[10] Wells TS, Sato P, Smith TC et al. Military Hospitalizations among deployed US service members following anthrax vaccination, 1998-2001. Human Vaccines 2006; 2: 54-59.

[11] Nass M. Data vs Conclusions in the Optic Neuritis Vaccination Investigation. Arch Neurol 2006; 63: 1809-10.

[12] Committee to Assess the Safety and Efficacy of the Anthrax Vaccine. Medical Follow Agency, Institute of Medicine. Anthrax Vaccine: Is it Safe?  Does it Work? National Academy Press 2002; Washington, DC. Appendix G.

[13] Committee to Assess the Safety and Efficacy of the Anthrax Vaccine. Medical Follow Agency, Institute of Medicine. Anthrax Vaccine: Is it Safe?  Does it Work? National Academy Press 2002; Washington, DC.

[14] Committee to Review the CDC Anthrax Vaccine Safety and Efficacy Research Program. Assessment of the CDC Anthrax Vaccine Research Program. Institute of Medicine 2003: National Academies Press. Washington, DC.

[15] Johannes L. Anthrax Vaccine May Increase Incidence Of Birth Defects For Pregnant Women. Wall Street Journal. January 16, 2002.

[16] Op cit. Committee to Assess the Safety and Efficacy of the Anthrax Vaccine. Medical Follow Agency, Institute of Medicine. Anthrax Vaccine: Is it Safe?  Does it Work? National Academy Press 2002; Washington, D.C. chapter 6. (http://www.nap.edu/openbook/0309083095/html/171.html and the following page)

[17] http://www.fda.gov/ohrms/dockets/dockets/80n0208/80n-0208-c000019-03-vol142.pdf

[18] http://www.defenselink.mil/releases/2002/b01162002_bt026-02.html

[19] MMWR 127 vol 51 No. 6. Notice to Readers: Status of US Department of Defense preliminary evaluation of the association of anthrax vaccination and congenital anomalies. http://www.cdc.gov/mmwr/preview/mmwrhtml/mm5106a5.htm

[20] Op. cit. Wiesen AR, Littell CT. Relationship between prepregnancy anthrax vaccination and pregnancy and birth outcomes among US Army women. JAMA. 2002 Mar 27;287(12):1556-60.

[21] Bush RA, Smith TC, Honner WK, Gray GC. Active surveillance of birth defects among U.S. Department of Defense beneficiaries: a feasibility study. Mil Med. 2001 Feb;166(2):179-83.

[22] http://www.anthrax.osd.mil/resource/qna/qaAll.asp?cID=312  ÒIn the March 27, 2002, issue of the Journal of American Medical Associations, two Army physicians published their peer-reviewed findings that women get pregnant at the same rate, whether anthrax-vaccinated or unvaccinated. These physicians from Fort Stewart, Georgia, also showed that women deliver offspring at the same rate, whether anthrax-vaccinated or unvaccinated. The Fort Stewart study found no difference in birth defect rates, either, but the study may have been too small to detect small differences. http://jama.amaassn.org/content/vol287/issue12/index.dtl Ò

[23] Partnership for Anthrax Vaccine Information. Muhiuddin Haider at George Washington University is the principal, under contract to the vaccine manufacturer, Bioport. www.gwu.edu/~cih/newsletter/CGHNewsletter11-2003.pdf http://www.gwu.edu/~cih/anthraxinfo/vaccine/vaccine_myths.htm#7

ÒMyth: The vaccine causes miscarriages and infertility

Fact:    There is no scientific information to support this statement. A recent study reported in the Journal of the American Medical Association followed over 4,000 military women to look at pregnancy rates and adverse birth outcomes between groups of women who had been vaccinated and women who had not. Although the study was small, it found that anthrax vaccination had no effect on pregnancy or adverse birth outcomes

[24] Wasserman G. Tripler Army Medical Center Survey. First Annual Department of Defense Conference for Biological Warfare Defense Immunizations. Fort Detrick, Maryland. May 26, 1999. transcript.

[25] GAO. Medical Readiness: Issues Concerning the Anthrax Vaccine. July 21, 1999. GAO/T-NSIAD-99-226